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KMID : 0351819980390020183
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Kyunpook University Medical Journal
1998 Volume.39 No. 2 p.183 ~ p.191
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Acute Perturbation of Epidermal Barrier Stimulates Type I Collagen mRNA Expression in the Skin of Hairless Mice
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Kim Do-Won
Kim Tae-Hoon
Shin Dong-Ju
Seo Jung-Min
Kim Jeong-Chul
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Abstract
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Background :Acute disruption of the epidermal permeability barrier by organic solvents or tape stripping elicits a homeostatic repair response in the epidermis which includes the immediate secretion of lamellar bodies, an increase in epidermal lipid synthesi:~, and extracellulaz remodeling of secreted lamellaz body-derived lipids, ultimately leading to the restoration of lipid-enriched membranes in the stratum corneum interstices. In addition, acute disruption o~F the barrier increases in epidermal DNA synthesis and cytokine production in the epidermis. It raises questions about the role of dermcepidermal interactions in barrier homeostasis.
Objective : To investigate the effect of acute perturbatitm of epidermal barrier on the dermis, we evaluated mRNA expression of type I collagen in the skin of hairless mice and observed dermal changes histopathologically.
Methods : To produce acute perturbation of epidermal barrier, the flank skin of 6-10 weeks old hairless mice were snipped with tape until TEWL levels reached 30g/m2h(mild perturbation) or 90g/m2h(modetate perturbation). At 10 hours, 5 days and 10 days after acute perturbation of epidermal barrier, Northern blot hybridization was performed to evaluate mRNA expression of type I collagen in the dermis and light microscopic examinations to observe dermal change.
Results :The expressions of type I collagen mRNA in the skin of hairless mice after 5 days in both state of mild and moderate perttbation of the barrier were increased compared with untreated skin. Light microscopic fmdings of 5 and 10 days after treatment show epidermal proliferation with 3-} granular layers and 2-3 spinous layers in all treated skin compazed with control skin with 1 granular layer and 1-2 spuiotu layers. The dermal findings of 5 and 10 days after treatment show increased number of fibroblasts and thickening of collagen fibers.
Conclusions : Present study indicates that acute disruption of epidermal permeability barrier with tapf: stripping may induce a variety of biologic responses in the underlying dermis including increased type I collagen synthesis. Further studies will be needed to evaluate the dermcepidermal interactions in barrier homeostasis.
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KEYWORD
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tape stripping, Epidermal barrier, TypeⅠcollagen, mRNA expression
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